CCK8/mtt/cell proliferation assay
Cell proliferation is the basis of organism growth, development, reproduction and heredity. For tumor research, cell proliferation is closely related to cell apoptosis, cell cycle and other phenotypes, and it is also one of the important contents of molecular biology and pharmacology research. MTT or CCK8 is a commonly used method to detect cell proliferation, which can be used to study the toxic effect of drugs on cells, or to explore the effect of overexpression or interference of a gene in cells on cell proliferation, so as to provide a basis for further study of gene function. The principle of MTT technology is that MTT (thiazole blue) can pass through the cell membrane and enter the cell. Dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to blue-purple crystals insoluble in water and deposit them in the cell, while dead cells do not have this function. Crystals can be dissolved in DMSO, and their light absorption value can be measured at 490nm wavelength by enzyme-linked immunosorbent detector, this indirectly reflects the number of living cells (within a certain range, the higher the optical density value, the stronger the cell viability). The principle of CCK8 technology is that the WST-8 in the CCK-8 reagent can be reduced to a highly water-soluble yellow product by dehydrogenase in the mitochondria of living cells under the action of the electron carrier 1-methoxy-5-methylphenazinium dimethyl sulfate (1-MethoxyPMS). The light absorption value of the enzyme-linked immunosorbent assay at 450nm wavelength can indirectly reflect the number of living cells (within a certain range, the amount of yellow product generated is proportional to the number of living cells).
Technical principle
Cell proliferation is the basis of organism growth, development, reproduction and heredity. For tumor research, cell proliferation is closely related to cell apoptosis, cell cycle and other phenotypes, and it is also one of the important contents of molecular biology and pharmacology research. MTT or CCK8 is a commonly used method to detect cell proliferation, which can be used to study the toxic effect of drugs on cells, or to explore the effect of overexpression or interference of a gene in cells on cell proliferation, so as to provide a basis for further study of gene function. The principle of MTT technology is that MTT (thiazole blue) can pass through the cell membrane and enter the cell. Dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to blue-purple crystals insoluble in water and deposit them in the cell, while dead cells do not have this function. Crystals can be dissolved in DMSO, and their light absorption value can be measured at 490nm wavelength by enzyme-linked immunosorbent detector, this indirectly reflects the number of living cells (within a certain range, the higher the optical density value, the stronger the cell viability). The principle of CCK8 technology is that the WST-8 in the CCK-8 reagent can be reduced to a highly water-soluble yellow product by dehydrogenase in the mitochondria of living cells under the action of the electron carrier 1-methoxy-5-methylphenazinium dimethyl sulfate (1-MethoxyPMS). The light absorption value of the enzyme-linked immunosorbent assay at 450nm wavelength can indirectly reflect the number of living cells (within a certain range, the amount of yellow product generated is proportional to the number of living cells).
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Changsheng medical animal modeling platform provides outsourcing services for animal disease modeling for customers, and can undertake the establishment of animal models related to various basic medical research, such as tumor, liver disease, respiratory disease, nervous system disease, cardiovascular disease, urinary system disease, endocrine disease, orthopedic disease, immune system disease, blood disease, gynecological disease, skin disease and other animal models. The modeling process supports field trips to ensure the quality and reliability of animal experiments.
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cell experiment
We provide an advanced cell experiment platform, which can detect the proliferation of MTT, CCK8, Edu and other cells, and can detect the invasion and migration ability of cells. We also provide experimental services such as plate cloning, soft agar cloning and comet assay, which can develop drug-resistant strains, construct cell lines and detect the function of tumor cells as needed. In addition, we offer an extensive library of cell resources that you can purchase to make your research results more reliable.
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Our pathology platform provides customers with comprehensive biological sample processing services. You can entrust us to carry out paraffin section, section scanning, fluorescence scanning, HE staining, and various special staining items, such as Masson, oil red O, PAS, safranin O, ALP, TRAP, toluidine blue, Tunel staining, methanol Congo red staining, safranin solid green staining, TTC staining, immunohistochemistry/fluorescence, in situ hybridization, etc.
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expression detection
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