DNA Methylation Detection
DNA methylation detection is a detection method that uses a variety of methods to determine the degree of methylation in malignant tumor cells in order to better judge the degree and spread of the lesion. DNA methylation is an important mechanism to regulate gene expression. The state of methylation is not invariable in the development of malignancy. Genome-wide hypomethylation in tumor cells is closely related to disease progression, tumor size and malignancy. Therefore, this test is very important for judging the degree of malignancy of the tumor.
Technical principle
NA methylation is an important mechanism of gene expression regulation, and DNA methylation detection is the use of a variety of methods to determine the level of DNA methylation in tumor cells. In the development of malignant tumors, the degree of methylation is not static, and the degree of hypomethylation in tumor cells is closely related to the deterioration of the disease, tumor volume and the degree of malignancy. Therefore, DNA methylation detection is very important for the evaluation of tumor malignancy. Methylation-specific PCR(MSP) is a specific methylation PCR method proposed by Herman et al. in 1996. The method utilizes sulfite treatment of genomic DNA to convert unmethylated cytosines to uracil, while methylated cytosines are unaffected; primers specific for methylated and unmethylated sequences are then designed for PCR. The MSP amplification product is detected by electrophoresis. If the primer for the methylated DNA chain can amplify the fragment, it indicates that the site is methylated; otherwise, it indicates that the detected site is not methylated. The MSP method has a wide range of applications and can detect whether specific sites are methylated. Bisulfite sequencing PCR(BSP), a bisulfite-modified sequencing method, was proposed by Frommer et al. in 1992. The method has high reliability and high accuracy, and can determine the methylation status of each CpG site in the target fragment. Treatment of genomic DNA with bisulfite converts unmethylated cytosines to uracil, while methylated cytosines are unaffected. BSP primers are then designed for PCR, during which all uracil is converted to thymine. Finally, the PCR product is sequenced to determine whether the CpG site is methylated. In addition, the use of cloning technology can improve the success rate of sequencing, this method is called BSP-cloning sequencing. The BSP method is highly accurate and can accurately detect the percentage of methylation.
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Plasma experiment service
Cold atmospheric plasma (CAP) is an emerging tumor treatment method that has been shown to be selective for many types of cancer cells, providing new opportunities for effective treatment of various cancers. CAP is a plasma generated at room temperature and atmospheric pressure, and has shown great potential for biomedical applications due to a favorable combination of reactive physical and chemical species, such as UV radiation, electrons, free radicals, ions, and excited molecules. Recently, it has been found that plasma activated medium (PAM) produced by exposure of aqueous media to CAP is as effective as direct CAP in inhibiting cancer cells, thereby increasing the range and flexibility of plasma-based therapy.
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Electrophysiological Experiment Service
Electrophysiological platform: The brain slice patch clamp system consists of amplifier, digital-to-analog converter, micro-operation, electrode drawing instrument, microscope and perfusion system. With a special glass micropipette adsorbed on the cell surface membrane, and then the implementation of the membrane voltage clamp, can measure the current of the magnitude of pA generated by the opening of a single ion channel. It can also be used to detect the cell excitability (action potential and model index), ion channel (potassium sodium calcium ion) and synaptic transmission (excitatory synaptic transmission and inhibitory synaptic transmission) in the brain slice of the desired site of drug/experimental treatment.
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Single Cell Sequencing
The single-cell sequencing platform provides you with comprehensive single-cell multi-omics services, including whole transcriptome (WTA) analysis, targeted transcriptome (TTA) analysis, full-length analysis of human V(D)J, full-length analysis of mouse V(D)J, and VDJ CDR3 analysis. Our protein group has 500 commercial Abseq antibodies: covering human/mouse immune-related Marker, AbSeq (human) immune detection combination, 30 kinds of personal immune Marker; At the same time, it provides multi-sample detection, including 12 kinds of human multi-sample labels, 12 kinds of mouse multi-sample labels, 6 kinds of nuclear multi-sample labels, genome scATAC-seq detection and CRISPR/Cas9 detection. At present, our single-cell sequencing platform involves many fields such as tumor typing, targeted drug use, immunotherapy, animal and plant embryonic development, and the occurrence and development mechanism of cardiovascular diseases. No matter what field you are engaged in, our services can meet your needs and provide comprehensive support for your research.
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model animal
Changsheng medical animal modeling platform provides outsourcing services for animal disease modeling for customers, and can undertake the establishment of animal models related to various basic medical research, such as tumor, liver disease, respiratory disease, nervous system disease, cardiovascular disease, urinary system disease, endocrine disease, orthopedic disease, immune system disease, blood disease, gynecological disease, skin disease and other animal models. The modeling process supports field trips to ensure the quality and reliability of animal experiments.
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cell experiment
We provide an advanced cell experiment platform, which can detect the proliferation of MTT, CCK8, Edu and other cells, and can detect the invasion and migration ability of cells. We also provide experimental services such as plate cloning, soft agar cloning and comet assay, which can develop drug-resistant strains, construct cell lines and detect the function of tumor cells as needed. In addition, we offer an extensive library of cell resources that you can purchase to make your research results more reliable.
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tissue staining
Our pathology platform provides customers with comprehensive biological sample processing services. You can entrust us to carry out paraffin section, section scanning, fluorescence scanning, HE staining, and various special staining items, such as Masson, oil red O, PAS, safranin O, ALP, TRAP, toluidine blue, Tunel staining, methanol Congo red staining, safranin solid green staining, TTC staining, immunohistochemistry/fluorescence, in situ hybridization, etc.
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expression detection
We have a first-class expression detection platform, can provide real-time fluorescence quantitative PCR, DNA methylation detection, Western blot, ELISA, SNP and mycoplasma detection and other professional services,. Our testing services cover the whole country, providing the perfect solution for the outsourcing of biomedical experiments. Our commitment is to provide customers with 100 percent true and reliable service, sign relevant legal agreements with customers, and provide permanent after-sales service.