real-time fluorescence quantitative PCR
Real-time fluorescence quantitative PCR is a cell molecular biology technology, which adds a fluorescent group that can specifically bind to the DNA product in the PCR reaction system, and realizes the real-time monitoring and quantification of the PCR reaction by real-time monitoring the accumulation of fluorescent signals in the reaction. This technology is mainly used in a variety of cell analysis, such as gene expression analysis, fragment analysis, mutation analysis and so on. Methods commonly used in qPCR detection include dye method and TaqMan probe method, which can be used to quantitatively or qualitatively determine the background expression of each sample.
Technical principle
Real-time fluorescent quantitative PCR(qPCR) is a cell molecular biology technology with innovative thinking. It introduces fluorescent groups that specifically bind to DNA products into the PCR reaction system, and uses the real-time accumulation of fluorescent signals to monitor the entire PCR reaction process. Finally, through relative or absolute quantification, we can determine the gene expression level or other molecular indicators of different samples. In qPCR detection, two commonly used methods are dye method and TaqMan probe method. The core of the TaqMan probe method is the probe molecule, which is usually a single strand of DNA. The 5 'end of the probe molecule is attached to a luminescent group and the 3' end is attached to a quencher group. When the probe molecule is intact and not hydrolyzed, we cannot detect the fluorescent signal from the luminescent group because the quenching group will absorb the fluorescence and extinguish it. When the PCR reaction begins, the template strand undergoes thermal denaturation to melt, forming a single strand, the TaqMan probe preferentially anneals to the template strand, and then the primer will subsequently anneal to the template, and the PCR reaction will undergo strand extension. In the process of extension, Taq enzyme will play a 5 '-3' exonuclease activity, and when it encounters a probe, it will cut the probe base one by one, so that the luminescent group and the quenching group are separated, and the fluorescence detection system can receive the fluorescence signal. Whenever a DNA strand is amplified, a fluorescent molecule is formed, and the accumulation of the fluorescent signal is synchronized with the formation of the PCR product. The specificity of the TaqMan probe method is not only provided by the primer, but also guaranteed by the probe molecule. Due to the higher annealing temperature of TaqMan probe, this method has better specificity. Multiple probes can be added to a reaction system to detect multiple genes at the same time.
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Plasma experiment service
Cold atmospheric plasma (CAP) is an emerging tumor treatment method that has been shown to be selective for many types of cancer cells, providing new opportunities for effective treatment of various cancers. CAP is a plasma generated at room temperature and atmospheric pressure, and has shown great potential for biomedical applications due to a favorable combination of reactive physical and chemical species, such as UV radiation, electrons, free radicals, ions, and excited molecules. Recently, it has been found that plasma activated medium (PAM) produced by exposure of aqueous media to CAP is as effective as direct CAP in inhibiting cancer cells, thereby increasing the range and flexibility of plasma-based therapy.
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Electrophysiological Experiment Service
Electrophysiological platform: The brain slice patch clamp system consists of amplifier, digital-to-analog converter, micro-operation, electrode drawing instrument, microscope and perfusion system. With a special glass micropipette adsorbed on the cell surface membrane, and then the implementation of the membrane voltage clamp, can measure the current of the magnitude of pA generated by the opening of a single ion channel. It can also be used to detect the cell excitability (action potential and model index), ion channel (potassium sodium calcium ion) and synaptic transmission (excitatory synaptic transmission and inhibitory synaptic transmission) in the brain slice of the desired site of drug/experimental treatment.
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Single Cell Sequencing
The single-cell sequencing platform provides you with comprehensive single-cell multi-omics services, including whole transcriptome (WTA) analysis, targeted transcriptome (TTA) analysis, full-length analysis of human V(D)J, full-length analysis of mouse V(D)J, and VDJ CDR3 analysis. Our protein group has 500 commercial Abseq antibodies: covering human/mouse immune-related Marker, AbSeq (human) immune detection combination, 30 kinds of personal immune Marker; At the same time, it provides multi-sample detection, including 12 kinds of human multi-sample labels, 12 kinds of mouse multi-sample labels, 6 kinds of nuclear multi-sample labels, genome scATAC-seq detection and CRISPR/Cas9 detection. At present, our single-cell sequencing platform involves many fields such as tumor typing, targeted drug use, immunotherapy, animal and plant embryonic development, and the occurrence and development mechanism of cardiovascular diseases. No matter what field you are engaged in, our services can meet your needs and provide comprehensive support for your research.
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model animal
Changsheng medical animal modeling platform provides outsourcing services for animal disease modeling for customers, and can undertake the establishment of animal models related to various basic medical research, such as tumor, liver disease, respiratory disease, nervous system disease, cardiovascular disease, urinary system disease, endocrine disease, orthopedic disease, immune system disease, blood disease, gynecological disease, skin disease and other animal models. The modeling process supports field trips to ensure the quality and reliability of animal experiments.
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cell experiment
We provide an advanced cell experiment platform, which can detect the proliferation of MTT, CCK8, Edu and other cells, and can detect the invasion and migration ability of cells. We also provide experimental services such as plate cloning, soft agar cloning and comet assay, which can develop drug-resistant strains, construct cell lines and detect the function of tumor cells as needed. In addition, we offer an extensive library of cell resources that you can purchase to make your research results more reliable.
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tissue staining
Our pathology platform provides customers with comprehensive biological sample processing services. You can entrust us to carry out paraffin section, section scanning, fluorescence scanning, HE staining, and various special staining items, such as Masson, oil red O, PAS, safranin O, ALP, TRAP, toluidine blue, Tunel staining, methanol Congo red staining, safranin solid green staining, TTC staining, immunohistochemistry/fluorescence, in situ hybridization, etc.
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expression detection
We have a first-class expression detection platform, can provide real-time fluorescence quantitative PCR, DNA methylation detection, Western blot, ELISA, SNP and mycoplasma detection and other professional services,. Our testing services cover the whole country, providing the perfect solution for the outsourcing of biomedical experiments. Our commitment is to provide customers with 100 percent true and reliable service, sign relevant legal agreements with customers, and provide permanent after-sales service.