Mycoplasma detection
Mycoplasma is a prokaryotic microorganism without cell wall and is one of the smallest known extracellular microorganisms. It is known for its highly diverse morphology, which can be spherical, rod-shaped, filamentous, branched and so on. In order to detect the presence of mycoplasma, the scientists used a specific sequence design primers and specific amplification using PCR technology. PCR selectively replicates the target DNA when mycoplasma contamination is present, followed by detection using agarose electrophoresis. If the result is positive, it means that mycoplasma is present. On the contrary, if there is no mycoplasma contamination, PCR cannot be amplified, because there is no template for replication, and the results of agarose electrophoresis will be negative. This method is accurate and rapid, and provides a reliable means for the detection of mycoplasma.
Technical principle
In the field of prokaryotes, the base sequence of rRNA is highly conserved. However, the base sequences of DNA spacers (such as 16S and 23S spacers) encoding rRNA are very different among different biological species. In particular, in Mycoplasma organisms, the DNA sequences and lengths of these spacers are both relatively conserved and largely different. In order to amplify the DNA spacer region encoding 16S and 23S rRNA, we designed a pair of primers named F1/R1 and applied them to this preparation. Through this specific amplification reaction system, we can only amplify Mycoplasma DNA with high sensitivity and specificity. By designing primers to specific sequences of mycoplasma, we can specifically amplify the target DNA using PCR technology. When there is mycoplasma contamination, PCR will copy the target DNA and produce specific amplification products. Through agarose electrophoresis, we can observe positive results, that is, obvious bands appear. On the contrary, if there is no mycoplasma contamination, PCR cannot be amplified due to the lack of template, so the agarose electrophoresis results will show negative, that is, no band appears.
Real Experimental Research Hundreds of Detection Experiments 6 Experimental Platforms
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Plasma experiment service
Cold atmospheric plasma (CAP) is an emerging tumor treatment method that has been shown to be selective for many types of cancer cells, providing new opportunities for effective treatment of various cancers. CAP is a plasma generated at room temperature and atmospheric pressure, and has shown great potential for biomedical applications due to a favorable combination of reactive physical and chemical species, such as UV radiation, electrons, free radicals, ions, and excited molecules. Recently, it has been found that plasma activated medium (PAM) produced by exposure of aqueous media to CAP is as effective as direct CAP in inhibiting cancer cells, thereby increasing the range and flexibility of plasma-based therapy.
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Electrophysiological Experiment Service
Electrophysiological platform: The brain slice patch clamp system consists of amplifier, digital-to-analog converter, micro-operation, electrode drawing instrument, microscope and perfusion system. With a special glass micropipette adsorbed on the cell surface membrane, and then the implementation of the membrane voltage clamp, can measure the current of the magnitude of pA generated by the opening of a single ion channel. It can also be used to detect the cell excitability (action potential and model index), ion channel (potassium sodium calcium ion) and synaptic transmission (excitatory synaptic transmission and inhibitory synaptic transmission) in the brain slice of the desired site of drug/experimental treatment.
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Single Cell Sequencing
The single-cell sequencing platform provides you with comprehensive single-cell multi-omics services, including whole transcriptome (WTA) analysis, targeted transcriptome (TTA) analysis, full-length analysis of human V(D)J, full-length analysis of mouse V(D)J, and VDJ CDR3 analysis. Our protein group has 500 commercial Abseq antibodies: covering human/mouse immune-related Marker, AbSeq (human) immune detection combination, 30 kinds of personal immune Marker; At the same time, it provides multi-sample detection, including 12 kinds of human multi-sample labels, 12 kinds of mouse multi-sample labels, 6 kinds of nuclear multi-sample labels, genome scATAC-seq detection and CRISPR/Cas9 detection. At present, our single-cell sequencing platform involves many fields such as tumor typing, targeted drug use, immunotherapy, animal and plant embryonic development, and the occurrence and development mechanism of cardiovascular diseases. No matter what field you are engaged in, our services can meet your needs and provide comprehensive support for your research.
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model animal
Changsheng medical animal modeling platform provides outsourcing services for animal disease modeling for customers, and can undertake the establishment of animal models related to various basic medical research, such as tumor, liver disease, respiratory disease, nervous system disease, cardiovascular disease, urinary system disease, endocrine disease, orthopedic disease, immune system disease, blood disease, gynecological disease, skin disease and other animal models. The modeling process supports field trips to ensure the quality and reliability of animal experiments.
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cell experiment
We provide an advanced cell experiment platform, which can detect the proliferation of MTT, CCK8, Edu and other cells, and can detect the invasion and migration ability of cells. We also provide experimental services such as plate cloning, soft agar cloning and comet assay, which can develop drug-resistant strains, construct cell lines and detect the function of tumor cells as needed. In addition, we offer an extensive library of cell resources that you can purchase to make your research results more reliable.
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tissue staining
Our pathology platform provides customers with comprehensive biological sample processing services. You can entrust us to carry out paraffin section, section scanning, fluorescence scanning, HE staining, and various special staining items, such as Masson, oil red O, PAS, safranin O, ALP, TRAP, toluidine blue, Tunel staining, methanol Congo red staining, safranin solid green staining, TTC staining, immunohistochemistry/fluorescence, in situ hybridization, etc.
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expression detection
We have a first-class expression detection platform, can provide real-time fluorescence quantitative PCR, DNA methylation detection, Western blot, ELISA, SNP and mycoplasma detection and other professional services,. Our testing services cover the whole country, providing the perfect solution for the outsourcing of biomedical experiments. Our commitment is to provide customers with 100 percent true and reliable service, sign relevant legal agreements with customers, and provide permanent after-sales service.