PAS glycogen staining
Histologically, there is a common staining method called PAS staining, also known as periodic acid Schiff staining or glycogen staining. It is mainly used to display sugar and other polysaccharide substances. With periodic acid, the hydroxyl groups on two adjacent carbons in the carbohydrate molecule can be oxidized to aldehyde groups, and then the Schiff reagent reacts with the aldehyde groups to form a purple-red colored substance. This method is very helpful for studying the glycogen content and structure of cell tissues.
Technical principle
In the field of histology, there is a technique called PAS staining (Periodic Acid-Schiff stain), which is also called periodic acid Schiff staining or glycogen staining. This method is commonly used to display glycos and other polysaccharide substances. The principle of PAS dyeing is to oxidize the hydroxyl groups on two adjacent carbons in saccharides to aldehyde groups by periodic acid, and then use Schiff's reagent to react with the aldehyde groups to produce a compound that appears purplish red. PAS staining Glycogen staining is a common staining method in pathology. The 1,2-glycol groups in the sugars and related substances are oxidized by the oxidizing agent, converting them to dialdehydes. The aldehyde group then binds to the Schiff reagent to form a compound that is magenta and appears purple-red. PAS technology is widely used to display glycogen and other polysaccharide substances. In addition to showing glycogen, this staining method can also show neutral mucous substances, certain acidic substances, as well as cartilage, pituitary, mold, fungi, pigments, amyloid substances and basement membranes. Therefore, PAS staining method plays an important role in the field of biology.
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